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Using the temperature gradient which was proposed by continuously measuring flat steel box girder of Runyang Bridge, temperature effects of flat steel box girder were studied for Taizhou Bridge. With three temperature gradient models （JTG D60--2004 specification, BS5400 specification and the temperature gradient which was proposed in this paper）, the stress of control sections was calculated by finite element program ANSYS. The calculated result indicated that the temperature gradient that was put forward in JTG D60-2004 specification and BS5400 specification for calculating the stress of fiat steel box girder was not suitable to apply to fiat steel box girder. The temperature gradient on flat steel box girder which was proposed in this paper was reasonable.