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The crude and purified Lipase OF from Candida rugosa were immobilized on silica gel by adsorption or adsorption/cross linking. The effect of Tween 80 on the enantioselective hydrolysis of Ketoprofen ester by immobilized lipase was examined. It is found that Tween 80 is effective in improving both the catalytic efficiency and the enantioselectivity of the immobilized lipase, where the conversion increases 3～4 folds and the enantiomeric ratio of the purified lipase increases from 10 to >100. Stability of the immobilized lipase decreases in the presence of Tween 80. This problem, however, can be solved by further cross linking of the adsorbed enzyme with glutaraldehyde. After six circles of repeated use in batch reaction, the conversion decreased from 61% to 37% and the enantioselectivity of the lipase remains constant.
The crude and purified Lipase OF from Candida rugosa were immobilized on silica gel by adsorption or adsorption / cross linking. The effect of Tween 80 on the enantioselective hydrolysis of Ketoprofen ester by immobilized lipase was examined. It is found that Tween 80 is effective in Improving both the catalytic efficiency and the enantioselectivity of the immobilized lipase, where the conversion ratio 3 to 4 folds and the enantiomeric ratio of the purified lipase increases from 10 to> 100. Stability of the immobilized lipase decreases in the presence of Tween 80. This problem , however, could be solved by further cross linking of the adsorbed enzyme with glutaraldehyde. After six circles of repeated use in batch reaction, the conversion decreased from 61% to 37% and the enantioselectivity of the lipase remains constant.