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目的:通过体外实验研究普通喷砂酸蚀纯钛表面和亲水性喷砂酸蚀纯钛表面对成骨细胞增殖、分化等生物学行为的影响。方法:纯钛片表面分别采用光滑处理(smooth pretreated Ti,PT)、大颗粒喷砂酸蚀表面处理(sand-blasted,large-grit,acid-etched,SLA)及亲水性化学活化大颗粒喷砂酸蚀表面处理(chemically-modified SLA,modSLA/SLActive),在表面接种MC3T3-E1成骨细胞,采用MTT、碱性磷酸酶半定量测试以及茜素红染色检测其对成骨细胞增殖、分化的影响,并采用实时荧光定量PCR检测成骨细胞在不同材料表面骨功能基因表达的差异。应用SAS 9.0软件包对数据进行统计学分析。结果:与光滑钛表面相比,普通喷砂酸蚀钛表面能通过促进ALP、钙基质的分泌和成骨功能基因(Runx2、OSX、OCN和OPN)的表达而显著抑制成骨细胞增殖并促进其分化。在表面粗糙度的基础上增加亲水性,可使这一效应更加明显。结论:表面粗糙度和亲水性是影响成骨细胞生物学行为的重要因素,粗糙钛表面能显著抑制成骨细胞增殖,促进其分化,亲水性的粗糙钛表面促进成骨细胞分化的作用更加显著。
OBJECTIVE: To study the effects of pure sand-blasting pure titanium surface and hydrophilic sandblasting pure titanium surface on the biological behaviors such as proliferation and differentiation of osteoblasts in vitro. METHODS: The surface of pure titanium was treated with smooth pretreated Ti (PT), sand-blasted (large-grit, acid-etched, SLA) and hydrophilic chemically activated large particle spray Surface-modified MC3T3-E1 osteoblasts were treated with chemically modified SLA (modSLA / SLActive). MTT, alkaline phosphatase and Alizarin red were used to detect osteoblast proliferation and differentiation The effects of osteoblasts on the expression of osteogenic genes on different materials were detected by real-time fluorescence quantitative PCR. SAS 9.0 software package for statistical analysis of the data. Results: Compared with the smooth titanium surface, the conventional sandblasting titania surface can significantly inhibit osteoblast proliferation and promote the expression of ALP, calcium matrix secretion and osteogenic function genes (Runx2, OSX, OCN and OPN) Its differentiation. This effect can be made more apparent by adding hydrophilicity to the surface roughness. Conclusion: The surface roughness and hydrophilicity are the important factors affecting the biological behavior of osteoblasts. The rough titanium surface can significantly inhibit the proliferation of osteoblasts and promote the differentiation of osteoblasts on the surface of hydrophilic and rough titanium. More significant.