论文部分内容阅读
用透射电镜及电子探针x-射线显微分析法研究了电针、吗啡和Tb~(3+)镇痛期间,小鼠不同脑区亚微结构Ca~(2+)分布的改变。实验结果表明,电针、吗啡和Tb~(3+)都使小鼠导水管周围灰质和下丘脑的髓鞘、线粒体出现大量沉淀颗粒。在电针、吗啡和Tb~(3+)处理前预注钌红对在髓鞘上的沉淀颗粒没有明显影响,但使线粒体内沉淀颗粒显著减少。电子探针x-射线显微分析证明,这些沉淀颗粒的主要成份是钙。结果提示,从Ca~(2+)分布的改变看,电针,吗啡和Tb~(3+)的镇痛作用十分相似,三者的镇痛效应可能是通过神经细胞膜内、外Ca~(2+)的移动来调控的。
Transmission electron microscopy and electron probe x-ray microscopy were used to study the changes of subcellular Ca 2+ distribution in electroacupuncture, morphine and Tb 3+ anesthesia mice. Experimental results show that electroacupuncture, morphine and Tb ~ (3 +) all make a large amount of sediment particles in the myelin and mitochondria around the aqueduct and the hypothalamus. Pretreatment with ruthenium red before electroacupuncture, morphine and Tb ~ (3+) treatment had no significant effect on the precipitated particles on myelin but significantly reduced the precipitated particles in the mitochondria. Electron probe x-ray microscopy proved that the main component of these precipitated particles is calcium. The results suggest that the analgesic effects of electroacupuncture, morphine and Tb ~ (3+) are very similar from the changes of Ca ~ (2+) distribution. The analgesic effects of the three drugs may be mediated through the intracellular Ca ~ (2+) 2+) to regulate the movement.