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目的评价连翘苷对主动脉斑块形成的干预作用。方法采用Apo E-/-小鼠喂养西方膳食饲料的方法建立动脉粥样硬化模型,建模同时用连翘苷低、中、高三个剂量(5 mg·kg-1、15 mg·kg-1、45 mg·kg-1BW)进行灌胃干预,连续12周。12周后,比较各组主动脉斑块面积以及Ac LDL刺激下的骨髓来源巨噬细胞PPAR-r、ABCA1、ABCG1、SR-B1基因的表达情况。结果与模型对照组相比,连翘苷中、高剂量组的主动脉斑块面积减少(P<0.05),骨髓来源巨噬细胞PPAR-r、ABCA1、ABCG1、SR-B1基因表达均上调(P<0.01,P<0.05)。结论连翘苷具有一定的抗动脉粥样硬化作用,其机制可能与促进巨噬细胞胆固醇流出的途径有关。
Objective To evaluate the effect of forsythin on the formation of aortic plaque. Methods Apo E - / - mice were fed western diets to establish atherosclerosis model. At the same time, the three models were treated with low, medium and high doses of forsythin (5 mg · kg -1, 15 mg · kg -1 , 45 mg · kg-1BW) for 12 weeks. Twelve weeks later, the aortic plaque area and the expression of PPAR-r, ABCA1, ABCG1 and SR-B1 in bone marrow-derived macrophages stimulated by Ac LDL were compared. Results Compared with the model control group, the area of aortic plaque in middle and high doses of forsythin decreased (P <0.05), and the mRNA expressions of PPAR-r, ABCA1, ABCG1 and SR-B1 in bone marrow-derived macrophages were all increased P <0.01, P <0.05). Conclusion Forsythin has a certain anti-atherosclerotic effect, the mechanism may be related to the promotion of macrophage cholesterol outflow pathways.