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利用VIGS技术,在烟草中沉默已知的抗病信号传导关键基因SIPK、NDR1、SGT1、HSP90、NPR1、Rar1、EDS1、WRKY1,再瞬时表达马铃薯抗病基因和其相应的无毒基因RB-Avrblb1和R3a-AvR3a,根据过敏反应(Hypersensitive Response,HR)是否被阻断来研究RB和R3a介导的抗病信号传导。结果发现SGT1和HSP90基因沉默阻断了HR反应的发生,表明SGT1和HSP90是参与RB和R3a抗病信号传导途径过程中的关键基因。该技术体系的建立,为发现新晚疫病抗病相关基因的功能验证提供了一个高效的技术平台。
By using VIGS technique, we silenced the known resistance genes SIPK, NDR1, SGT1, HSP90, NPR1, Rar1, EDS1 and WRKY1 in tobacco and then transiently expressed the resistance genes of potato and its corresponding non-toxic gene RB-Avrblb1 And R3a-AvR3a were used to study RB and R3a-mediated resistance signaling according to whether Hypersensitive Response (HR) was blocked. The results showed that the silencing of SGT1 and HSP90 blocks the occurrence of HR reaction, indicating that SGT1 and HSP90 are involved in RB and R3a resistance signal transduction pathway in the key genes. The establishment of this technical system has provided an efficient technical platform for the functional verification of the resistance-related genes of new late blight disease.