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离体大鼠比目鱼肌分别在含０～６μｍｏｌ／Ｌ硝苯地平（ＮＦ）和１４Ｃ－葡萄糖（１４Ｃ－Ｇｌｕ）的ＫＲＢ液中温育，然后用不含１４Ｃ－Ｇｌｕ的ＫＲＢ液冲洗，每５分钟一次，共７５分钟，后２５分钟用含１０Ｕ／Ｌ胰岛素（Ｉｎｓ）的ＫＲＢ液冲洗。结果Ｉｎｓ使１４Ｃ－Ｇｌｕ的分段流量（λ）显著增高，说明Ｉｎｓ加速了葡萄糖在细胞内外的转运。ＮＦ浓度４μｍｏｌ／Ｌ和６μｍｏｌ／Ｌ两组在Ｉｎｓ负荷后，λ值显著低于０～２μｍｏｌ／Ｌ各组，提示高浓度的ＮＦ抑制Ｉｎｓ介导的葡萄糖在肌细胞的转运。
Isolated rat soleus muscle was incubated in KRB solution containing 0-6 μmol / L Nifedipine (NF) and 14C-Glu (14C-Glu) and then rinsed with 14C-Glu-free KRB solution every 5 minutes Once for a total of 75 minutes, and 25 minutes later rinsed with KRB solution containing 10 U / L insulin (Ins). Results Ins significantly increased the fractional flow (λ) of 14C-Glu, indicating that Ins accelerates glucose transport both inside and outside the cell. The values of λ in 4μmol / L and 6μmol / L NF groups were significantly lower than those in 0 ~ 2μmol / L group after Ins load, suggesting that high concentration of NF inhibited Ins-mediated glucose transport in muscle cells.