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实验用近亲繁殖的雌性C 57 B 1/6小鼠,8～10周龄,体重18～20 g。采用两种给镉(CdCl_2)方式:(1)一次吸入镉(Cd)气溶胶共60分钟,浓度为0.88 ng/m~3,气溶胶颗粒直径至少一半为0.3～0.7μm(平均空气动力学直径=0.7±0.3μm),吸入后1、4、7和14天,静脉注射绵羊红细胞(SRBC),对照是在1和14天静脉注射SRBC。(2)饮用含镉水,浓度为5、100和200 ppm,共12～16周,静脉注射SRBC。均在免疫后4天取脾脏,检测有核细胞数、细胞生活力、空斑形成细胞
Inbred female C57B 1/6 mice, 8-10 weeks old, weighing 18-20 g, were used for experiments. Two approaches to cadmium (CdCl 2) were used: (1) one inhalation of a cadmium (Cd) aerosol for a total of 60 minutes at a concentration of 0.88 ng / m 3 and at least one half aerosol particle diameter of 0.3 to 0.7 μm (mean aerodynamic Diameter = 0.7 ± 0.3 μm). Sheep red blood cells (SRBC) were injected intravenously 1, 4, 7 and 14 days after inhalation and SRBC was injected intravenously on days 1 and 14. (2) Drinking cadmium-containing water at concentrations of 5, 100 and 200 ppm for 12 to 16 weeks intravenously with SRBC. Four days after immunization, the spleens were taken and the number of nucleated cells, cell viability, plaque-forming cells