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目的了解莱州市发热伴血小板减少综合症病毒(简称:新布尼亚病毒,SFTSV)在莱州市健康人群和家养动物中的流行特点,为疫情预防和控制提供依据。方法采用分层随机抽样方法,采集莱州市不同年龄组健康人群血清1 065份,用间接法ELISA检测SFTSV IgG抗体,分析不同年龄组、性别、职业间抗体阳性率的差异;采用随机抽样的方法,采集莱州家养动物(牛、羊、狗、猪、鸡)血清1 752份,用双抗原夹心法ELISA检测血清中SFTSV总抗体,对不同生长环境动物SFTSV抗体阳性率进行比较。结果共检测健康人群血清1 065份,SFTSV IgG抗体阳性率3.29%(35/1 065);5种家养动物血清1 752份,SFTSV总抗体阳性率36.64%(642/1 752),其中牛、羊、狗、猪、鸡血清样本SFTSV总抗体阳性率分别为48.50%、54.00%、24.73%、0.00%和55.17%。健康人群中不同性别和年龄组间抗体阳性率差异均无统计学意义(P>0.05)、不同职业间抗体阳性率差异有统计学意义(P<0.05);不同动物间SFTSV总抗体阳性率差异有统计学意义(P<0.01),散养动物与圈养动物血清SFTSV总抗体阳性率差异有统计学意义(P<0.01)。结论莱州市为SFTSV流行地区,健康人群和家养动物中均存在SFTSV的流行,且对SFTSV普遍易感,从事农业生产的劳动者感染风险较高,应加强自我防范意识。
Objective To understand the epidemiological features of fever and thrombocytopenia syndrome virus (SFBV) in Laizhou and healthy domestic animals in Laizhou City, and provide a basis for the prevention and control of the epidemic. Methods Stratified random sampling method was used to collect 1 065 serum samples from healthy population of different age groups in Laizhou City. SFTSV IgG antibody was detected by indirect ELISA. The positive rates of antibody in different age groups, sexes and occupations were analyzed. By using random sampling method , Collected 1 752 serums from Laizhou domestic animals (cattle, sheep, dogs, pigs and chickens). The total SFTSV antibody in serum was detected by double antigen sandwich ELISA, and the positive rates of SFTSV antibodies in different growth environment animals were compared. RESULTS: A total of 1 065 serum samples were obtained from healthy individuals. The positive rate of SFTSV IgG antibody was 3.29% (35/1 065). The average serum SFTSV antibody was 1,752 (36.64%, 642/1 752) The positive rates of total SFTSV antibody in sheep, dog, pig and chicken serum samples were 48.50%, 54.00%, 24.73%, 0.00% and 55.17%, respectively. There was no significant difference in the positive rate of antibody between different sexes and age groups in healthy population (P> 0.05), and the positive rate of antibody among different occupations was statistically significant (P <0.05). The positive rate of total antibody to SFTSV in different animals (P <0.01). There was a significant difference in the positive rate of SFTSV total antibody between the free-range animals and the captive animals (P <0.01). Conclusion In SFRSV epidemic area, SFTSV prevalence was found in both healthy population and domesticated animals, and it was generally susceptible to SFTSV. Laborers engaged in agricultural production were at a higher risk of infection, and self-prevention awareness should be strengthened.