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本文报道应用逆转录PCR检测角蛋白K19基因表达,以检查乳腺癌腋窝淋巴结微转移灶的方法。作者们从18个癌症病人体内获取了57个淋巴结标本,每个淋巴结一分为二,一半进行组织学和免疫组化检查,另一半用液氮速冻,-70℃保存用于PCR检测。常规组织学检查和采用抗K19抗体的免疫组化检查结果都发现57个淋巴结标本中有18个肿瘤微转移灶。这18个淋巴结mRNA逆转录PCR扩增产物中全部检测到长度为460bp的K19片断。在组织学检查阴性的39个样本中,PCR扩增,经EB染色后有4个为阳性(10％),而进一步杂交后又发现10例阳性(28％)。
This article reported the application of reverse transcription PCR to detect the expression of keratin K19 gene to examine the micrometastasis of axillary lymph nodes in breast cancer. The authors obtained 57 lymph node specimens from 18 cancer patients. Each lymph node was divided into two groups and half were examined for histology and immunohistochemistry. The other half was snap frozen with liquid nitrogen and stored at −70° C. for PCR. Routine histological examination and immunohistochemical examination using anti-K19 antibody revealed that there were 18 tumor micrometastases in 57 lymph node specimens. A total of 460 bp K19 fragments were detected in all 18 lymph node mRNA RT PCR amplification products. Of the 39 samples negative for histological examination, 4 were positive by PCR amplification (40%) after EB staining, and 10 were positive (28%) by further hybridization.