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目的观察ＥＢＶ┐ＢＨＲＦ１基因阳性（ＳＵＮＥ┐１、Ｒａｊｉ和Ｂ９５┐８）与阴性（Ｋ５６２、ＹＡＣ）细胞株对３种诱导细胞凋亡（ａｐｏｐ┐ｔｏｓｉｓ）因素（无血清培养４８小时，４３℃作用１０分钟或２×１０－６ｍｏｌ／Ｌ地塞米松作用２４小时）的抵抗力差异。方法ＰＣＲ扩增ＥＢＶ┐ＢＨＲＦ１基因片段诱导后经电镜及电泳ＤＮＡ检测细胞凋亡。结果分别从Ｂ９５┐８、Ｒａｊｉ及人低分化鼻咽癌细胞株（ＳＵＮＥ┐１）ＤＮＡ中扩增出ＥＢ病毒的ＢＨＲＦ１全基因片段，而Ｋ５６２、ＹＡＣ细胞株则不含该基因片段。分别经上述３种方法处理后，前３种细胞株的形态及ＤＮＡ电泳行为未发生明显改变，而后２种细胞株则出现典型的细胞凋亡特征，即细胞核凝缩，细胞膜及细胞器基本保持完整；ＤＮＡ电泳出现梯形（Ｌａｄｄｅｒ）条带。结论初步揭示ＢＨＲＦ１基因产物在抑制上述因素所诱导的细胞凋亡方面起着重要作用
Objective To investigate the effects of EBV┐BHRF1 gene positive (SUNE┐1, Raji and B95┐8) and negative (K562, YAC) cell lines on three kinds of apoptosis-inducing factors (48 hours without serum and 43°C) The resistance difference was 10 minutes or 2×10-6 mol/L dexamethasone for 24 hours). Methods The EBV/BHRF1 gene fragment was amplified by PCR and apoptosis was detected by electron microscopy and electrophoresis. Results The BHRF1 gene fragment of Epstein-Barr virus was amplified from the DNA of B95┐8, Raji and human poorly differentiated nasopharyngeal carcinoma cell line (SUNE┐1), while the K562 and YAC cell lines did not contain the gene fragment. After treatment with the above three methods, the morphology and DNA electrophoresis behavior of the first three cell lines did not change significantly, while the latter two cell lines exhibited typical cell apoptosis characteristics, ie, cell nuclei condense, and cell membranes and organelles remained basically intact. ; DNA electrophoresis appeared ladder (Ladder) band. Conclusion It is initially revealed that BHRF1 gene product plays an important role in inhibiting apoptosis induced by the above factors.