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目的:探讨细胞周期调控因子在大肠癌中的表达及其与大肠癌临床病理特征的关系。方法:应用免疫组化SP法对70例大肠癌组织及距癌灶3cm以外的癌旁组织、10cm以外的正常组织中CyclinD1和p16进行检测。结果:CyclinD1在大肠癌中过度表达为36/70(51.4%)并与肿瘤的分化程度呈反比,有淋巴结转移的大肠癌,其CyclinD1的阳性率为70.0%,无淋巴结转移的大肠癌阳性率为44.0%,两者相比差异有显著性(P<0.05)。p16在大肠癌中为低表达33/70(47.1%),癌旁组织和正常组织中p16的表达分别为57.1%和71.4%。CyclinD1与p16呈负相关关系(P<0.05)。结论:CyclinD1的过度表达与肿瘤的分化程度、淋巴结转移密切相关;CyclinD1的过度表达和p16的低表达在大肠癌发生中起协同作用;大肠癌的发生机制涉及CyclinD1和p16调节环路中多个基因的异常。
Objective: To investigate the expression of cell cycle regulators in colorectal cancer and its relationship with the clinicopathological features of colorectal cancer. Methods: Immunohistochemical SP method was used to detect the expression of CyclinD1 and p16 in 70 cases of colorectal cancer tissues and adjacent non-cancerous tissues 3cm away from the tumor and normal tissues 10cm away. Results: The overexpression of CyclinD1 in colorectal carcinoma was 36/70 (51.4%), which was inversely correlated with the degree of differentiation. The positive rate of CyclinD1 in colorectal cancer with lymph node metastasis was 70.0%. The positive rate of colorectal cancer without lymph node metastasis Was 44.0%, there was significant difference between the two (P <0.05). The expression of p16 in colorectal carcinoma was 33/70 (47.1%) low, while the expression of p16 in adjacent tissues and normal tissues was 57.1% and 71.4% respectively. CyclinD1 was negatively correlated with p16 (P <0.05). Conclusion: The overexpression of CyclinD1 is closely related to tumor differentiation and lymph node metastasis. The overexpression of CyclinD1 and the low expression of p16 play a synergistic role in the carcinogenesis of colorectal cancer. The mechanism of colorectal cancer involves multiple CyclinD1 and p16 regulatory loops Gene abnormalities.