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目的 通过分析麻醉状态和清醒状态小鼠在相同热休克条件下的热休克反应异同 ,了解整体调控在生物热休克反应中的意义和作用。 方法 经 4 0、4 2、4 4和 4 6℃ ,分别处理清醒状态和麻醉状态小鼠 30min ,于正常饲养条件下恢复 2 4h后 ,检测其肝脏热休克蛋白 70 (HSP70 )的表达差异及酸性和中性蛋白水解酶活性变化。 结果 当热休克温度为 4 4～ 4 6℃时 ,清醒状态小鼠肝脏的HSP70合成能力下降 ,而麻醉小鼠肝脏保持较强的HSP70合成能力 ;两组小鼠肝脏的酸性蛋白水解酶活性基本与HSP70合成正相关 ,中性蛋白水解酶活性基本与HSP70合成负相关。 结论 麻醉状态小鼠肝脏的热休克耐受性大于清醒小鼠 ;小鼠肝脏的热休克反应受整体和细胞两个水平调控 ,并涉及除HSP70合成以外的其他生化活动
OBJECTIVE: To understand the significance and role of overall regulation in the biological heat shock response by analyzing the similarities and differences of heat shock response in anesthetized and awake mice under the same heat shock conditions. Methods The mice in awake and anesthetized mice were treated for 30 min at 4, 4, 4, 4, and 46 ℃, respectively, and then recovered for 24 hours under normal conditions. The expression of heat shock protein 70 (HSP70) Changes in acid and neutral proteolytic enzyme activities. Results When the heat shock temperature was 44 ~ 46 ℃, the HSP70 synthesis ability of the awake mice liver decreased, while the liver of the anesthetized mice maintained a strong ability of HSP70 synthesis. The liver acidic proteolytic activity Positive correlation with HSP70 synthesis, neutral proteolytic enzyme activity and HSP70 synthesis negative correlation. Conclusions Heat shock tolerance in anesthetized mice is greater than that in awake mice; the heat shock response in mouse liver is regulated both at the whole and in cells, and involves biochemical activities other than HSP70 synthesis