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2009年4月,从河北邯郸、邢台、保定麦田采集表现小麦丛矮病症状的植株,经室内人工饲养的无毒灰飞虱饲食传毒,可致健康小麦表现叶脉间褪绿,矮化,分蘖增多,整株黄化等典型丛矮病症状。根据已报道的小麦丛矮病毒(Wheat ro-sette stunt virus,WRSV)各基因片段和北方禾谷花叶病毒(Northern cereal mosaic virus,NCMV)基因组序列设计引物,利用反转录PCR(RT-PCR)方法,在上述3个标样中均检测到了NCMV,而未检测到WRSV。用RT-PCR扩增到NCMV全基因组序列,长度为13221nt,具有9个开放读框。核酸序列比对结果显示,该序列与日本报道的NCMV有93%的同源性;推导氨基酸序列与日本报道的NCMV有99%同源性;但核酸和氨基酸序列与WRSV无明显同源性。上述结果表明,从河北省小麦丛矮病株中检测到的病原是NCMV,首次通过分子生物学研究检测到中国小麦上感染有NCMV。
April 2009, from Handan, Hebei, Xingtai, Baoding wheat field show symptoms of wheat clumpy dwarf disease plants, indoor artificial feeding of non-toxic Laodelphas feeding poisoning, can cause healthy wheat chlorotic chlorotic, dwarfed , Tillering increased, whole plant yellow typical dwarf symptoms. Primers were designed according to the published gene sequences of wheat ro-sette stunt virus (WRSV) and northern cereal mosaic virus (NCMV). Reverse transcription PCR (RT-PCR) ) Method, NCMV was detected in the above three standard samples, but no WRSV was detected. The entire genome sequence of NCMV was amplified by RT-PCR and was 13221nt with 9 open reading frames. Nucleotide sequence alignment showed that this sequence shared 93% homology with the NCMV reported in Japan. The deduced amino acid sequence was 99% homologous to the NCMV reported in Japan. However, the nucleotide and amino acid sequence showed no significant homology with WRSV. The above results showed that NCMV was detected in wheat dwarf strain of Hebei Province by NCMV, and NCMV was detected in Chinese wheat through molecular biology for the first time.