论文部分内容阅读
目的通过分析不同浓度盐胁迫下甘草酸积累量和总糖、粗蛋白、粗纤维、粗脂肪及灰分量的变化以及它们的相关性,研究盐胁迫对甘草酸积累的影响。方法采用不同质量浓度(3、6、9 mg/mL)NaCl溶液处理一年生盆栽甘草,分别于35、70、105 d取样,测定甘草酸量,总糖、粗蛋白、粗纤维、粗脂肪及灰分5种组分的量,分析盐胁迫对各组分比例关系的影响及甘草酸量与各组分量的相关性。结果盐胁迫70 d时,6、9 mg/mL盐溶液处理组的甘草酸量显著高于对照(CK);6、9 mg/mL处理组的粗蛋白显著高于CK,而9 mg/mL处理组的总糖显著低于CK。盐胁迫105 d时,9 mg/mL处理组的粗脂肪显著高于CK;盐胁迫70 d和105 d时,9 mg/mL处理组的粗脂肪比例显著高于CK,但总糖比例明显低于CK;盐胁迫70 d和105 d时,甘草酸量与粗脂肪、灰分量呈正相关,与总糖量呈负相关。结论甘草酸的积累与粗蛋白、总糖量、粗脂肪、灰分量的分配密切相关,适当的盐胁迫可以刺激甘草内的糖代谢,加速物质的分解,促进甘草的次生代谢,使甘草酸形成并积累。
Objective To study the effects of salt stress on glycyrrhizic acid accumulation by analyzing the changes of glycyrrhizic acid accumulation, total sugar, crude protein, crude fiber, crude fat and ash content under salt stress and their correlation. Methods The annual pots of Glycyrrhiza uralensis Fisch were treated with different concentrations of NaCl solution (3, 6, 9 mg / mL), and the contents of glycyrrhizic acid, total sugar, crude protein, crude fiber, crude fat and ash were measured at 35, The effects of salt stress on the proportion of each component and the correlation between the content of glycyrrhiza and each component were analyzed. Results The content of glycyrrhizic acid in 6, 9 mg / mL saline solution was significantly higher than that in control (CK) at 70 d. The crude protein in 6, 9 mg / mL treatment group was significantly higher than that in CK, while 9 mg / mL Total sugar in the treatment group was significantly lower than CK. The crude fat of 9 mg / mL treatment group was significantly higher than that of CK at 105 d of salt stress. The crude fat percentage of 9 mg / mL treatment group was significantly higher than that of CK at 70 d and 105 d after salt stress, but the total sugar ratio was significantly lower At CK and salt stress for 70 days and 105 days, there was a positive correlation between licorice acid content and crude fat and ash content, but negatively correlated with total sugar content. Conclusion Glycyrrhizic acid accumulation is closely related to the distribution of crude protein, total sugar content, crude fat and ash content. Appropriate salt stress can stimulate glycometabolism in licorice, speed up the decomposition of substances and promote the secondary metabolism of licorice, Formation and accumulation.