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目的:观察芪蟾口服结肠靶向片浸膏对小鼠的急性毒性反应,以评价其安全性;研究芪蟾口服结肠靶向浸膏对H22荷瘤小鼠的抗肿瘤作用。方法:采用经典的急性毒性试验方法测定芪蟾口服结肠靶向浸膏的半数致死量(LD50),观察急性毒性症状,记录死亡数;建立H22荷瘤小鼠模型,随机分成6组,分别给予芪蟾口服结肠靶向浸膏高、中、低剂量灌胃,临床汤剂组灌胃,化疗药物5—Fu腹腔注射单独应用,生理盐水灌胃。正常饲养,给药10天后,摘眼球取血,处死小鼠,分别取出肿瘤组织、小鼠脾脏、胸腺,进行抑瘤率、脾指数、胸腺指数的检测。采用酶联免疫法测定TNF-α、IFN-γ细胞因子水平。结果:芪蟾口服结肠靶向片浸膏小鼠口服LD50为43.26g.kg-1,相当于人临床日用量的19.6倍,;芪蟾口服结肠靶向浸膏各剂量组均可有效抑制肿瘤生长,5-Fu腹腔注射单独应用的抑瘤率为40.52%,芪蟾靶向浸膏3个剂量组的抑瘤率分别为37.41%、33.21%、31.85%。与5-Fu组比较,芪蟾口服结肠靶向浸膏各剂量组能显著提高小鼠脾脏、胸腺的重量(P<0.05)。与模型对照组相比,能明显提高细胞因子TNF-α,IFN-γ的水平(P<0.05)结论:芪蟾靶向浸膏各剂量组均可不同程度抑制肿瘤的生长,以芪蟾靶向浸膏低剂量组效果最为明显,其作用机制与增加小鼠免疫器官重量、增加细胞因子TNF-α,IFN-γ水平等有关。提示芪蟾靶向浸膏有抗肿瘤作用,为临床应用提供科学的实验数据,对临床有实际的指导意义。
OBJECTIVE: To observe the acute toxicity of Qiaodong oral colon-targeted tablet extract in mice to evaluate its safety. To study the antitumor effect of Qiaodong oral colon-targeted extract on H22 tumor-bearing mice. Methods: The classical acute toxicity test was used to determine the LD50 of the oral administration of Radix Astragalus. The acute toxicity symptoms were observed and the number of death was recorded. H22 tumor-bearing mice model was established and randomly divided into 6 groups Astragalus oral colon-targeted extract of high, medium and low doses of gavage, clinical decoction group gavage, chemotherapy drug 5-Fu intraperitoneal injection alone, saline gavage. The mice were sacrificed and the tumor tissues, mouse spleen and thymus were removed, and the tumor inhibition rate, spleen index and thymus index were detected. Enzyme-linked immunosorbent assay was used to detect the levels of TNF-α and IFN-γ cytokines. Results: LD50 of oral LDH was 43.26g.kg-1, which was 19.6 times higher than that of human daily dose. The inhibitory rate of tumor growth was 40.52% when treated with 5-Fu intraperitoneal injection alone or 37.41%, 33.21% and 31.85% respectively with the three dose groups of Targeted toad. Compared with the 5-Fu group, each dose group of Radix Astragalus could increase the weight of spleen and thymus in mice (P <0.05). Compared with the model control group, the levels of TNF-α and IFN-γ in the cytokines were significantly increased (P <0.05). Conclusion: The doses of the stilbene-targeting extract can inhibit tumor growth in varying degrees. To extract low-dose group the most obvious effect, its mechanism of action and increase the weight of immune organs in mice, increased cytokines TNF-α, IFN-γ levels and so on. It is suggested that the anti-tumor effect of stilbene-targeting extract can provide scientific experimental data for clinical application, which has practical guiding significance to clinical practice.