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在pH 3.2的邻苯二甲酸氢钾-HCl缓冲液中,酸性铬兰K(ACBK)-OP与牛血清白蛋白(BSA)形成三元离子缔合物,导致共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)的显著增强,光谱最大散射波长分别位于420,678和340nm。体系的光散射强度与BSA浓度在一定范围内呈线性增强,RRS在0~3.5 mg/L,SOS在0~3 mg/L,FDS在0~3 mg/L范围内对BSA的检出限分别为0.3,0.7和0.8μg/L,据此建立了测定BSA的共振线性(RRS)和共振非线性光散射(RNLS)分析法。以RRS法考察了酸性铬兰K-OP与白蛋白形成三元缔合物的适宜条件、影响因素等。方法可用于合成样品及血清样品中蛋白含量的测定。
Acidic Chromium Blue K (ACBK) -OP forms ternary ion-association with bovine serum albumin (BSA) in pH 3.2 potassium hydrogenphosphate-HCl buffer resulting in Resonance Rayleigh Scattering (RRS), Secondary scattering (SOS) and frequency doubling scattering (FDS) were significantly enhanced with the maximum spectral scattering wavelengths at 420, 678 and 340 nm, respectively. The light scattering intensity and BSA concentration increased linearly with the range of 0 ~ 3.5 mg / L for RRS and 0 ~ 3 mg / L for SOS and 0 ~ 3 mg / L for FDS (RRS) and resonance non-linear light scattering (RNLS) methods for the determination of BSA were established at 0.3, 0.7 and 0.8 μg / L, respectively. The RRS method was used to investigate the suitable conditions, influencing factors and the like of the ternary association product between acid chrome blue K-OP and albumin. The method can be used for the determination of protein content in synthetic samples and serum samples.