干旱胁迫对持绿性高粱叶片渗透调节及叶绿体超微结构的影响

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在盆栽条件下,研究了开花期和灌浆期干旱胁迫(土壤含水量为田间最大持水量的45%~50%)对持绿性高粱(B35)和非持绿性高粱(三尺三)叶片水分、渗透调节物质以及叶绿体超微结构的影响.结果表明:干旱胁迫下,两高粱品系叶片自由水含量下降,束缚水含量增加,相对含水量降低,水分饱和亏缺增加,相对电导率增大,但三尺三各指标的变化幅度均大于B35.对于渗透调节物质,干旱胁迫下,三尺三可溶性糖含量的增幅大于B35,脯氨酸含量的增幅小于B35,可溶性蛋白含量的降幅大于B35.干旱胁迫下,B35与三尺三的叶绿体超微结构均受到一定程度的破坏,但B35叶绿体结构保持相对完好,受损程度明显小于三尺三.在干旱胁迫下,持绿性高粱通过较强的渗透调节表现出更好的干旱适应能力. Under potted condition, the effects of drought stress on the flowering and grain filling stages (soil water content was 45% ~ 50% of the maximum water holding capacity in the field) were studied on the leaves of holding green sorghum (B35) and non-green sorghum Osmotic adjustment substance and chloroplast ultrastructure.The results showed that under drought stress, the content of free water in leaves of two sorghum lines decreased, the bound water content increased, the relative water content decreased, the water saturation deficit increased, and the relative conductivity increased , But the changes of three-foot three indexes were all greater than B35. For osmotic adjustment substances, the increase of three-foot-three soluble sugar content was greater than B35 under drought stress, the increase of proline content was less than B35, and the decrease of soluble protein content was greater than that of B35 Under drought stress, the ultrastructure of chloroplast of B35 and three-foot three were destroyed to a certain degree, but the structure of chloroplast of B35 was relatively intact and the damage degree was obviously less than three-foot-three. Under the drought stress, Strong osmotic adjustment showed better drought adaptability.
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