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目的:建立兔慢性肝纤维化胆石病模型,研究大黄灵仙颗粒对慢性肝纤维化胆石病兔模型的干预作用。方法:参考既往造模方法进行动物模型构建并进行中药制剂大黄灵仙颗粒干预,用药12周后,麻醉后耳缘静脉采血并取肝脏组织,测定肝组织羟脯氨酸含量,HE染色观察肝脏组织病理变化及检测肝功能相关生化指标ALT、AST、TBIL、DBIL、IBIL、TC、TG。结果:与对照组相比,各大黄灵仙颗粒组,治疗组血清ALT、AST、TBIL、DBIL、IBIL、TC、TG等生化指标均较前明显改变(均为P<0.05);与对照组相比,各大黄灵仙颗粒组,治疗组肝组织羟脯氨酸含量均明显下降(P<0.01);病理显示各大黄灵仙颗粒组光镜下可见遭到破坏的肝组织逐渐修复,肝细胞排列略变规则,水肿肝细胞明显减少,炎性细胞及纤维组织减少,区域呈点状、片状坏死明显减少。结论:大黄灵仙颗粒能防治慢性肝纤维化胆石病,具体效应机制有待探索。
Objective: To establish a model of cholelithiasis in rabbits with chronic liver fibrosis and study the effect of rhubarb and Lingxian granule on the model of chronic liver fibrosis gallbladder in rabbits. Methods: The animal models were constructed according to the previous methods and the Chinese medicine rhubarb granules was intervened. After 12 weeks of treatment, the blood was drawn from the ear vein and the liver tissue was taken for measurement of hydroxyproline content in the liver tissue. HE staining was used to observe the liver Histopathological changes and detection of liver function related biochemical indicators ALT, AST, TBIL, DBIL, IBIL, TC, TG. Results: Compared with the control group, the biochemical indexes such as ALT, AST, TBIL, DBIL, IBIL, TC and TG of the treatment groups were significantly changed (all P <0.05) Compared with the control group, the content of hydroxyproline in the liver of the Huanghuangxian Granules group and the treatment group were significantly decreased (P <0.01). The pathology showed that the liver tissue of the Huanghuangxian granule group under the light microscope was gradually repaired and the liver Cell arrangement slightly change rules, edema hepatocytes decreased significantly, inflammatory cells and fibrous tissue decreased, the area was punctate, sheet necrosis was significantly reduced. Conclusion: Rhubarb granules can prevent and treat chronic hepatic fibrosis gallbladder disease, the specific mechanism needs to be explored.