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目的探讨中西医联合用药对脓毒症大鼠的影响及其影响机制,为临床早期诊断脓毒症和评估其预后提供依据。方法选取SPF级健康SD大鼠60只,采用随机数字表法分为5小组,每组各12只,包括正常对照组(A组)、脓毒症模型大鼠中的生理盐水对照组(B组)、西药组(C组)、中药组(D组)、中药联合西药组(E组);A组不造模,静脉注射生理盐水;其余均采用通过股静脉连续滴注脂多糖(LPS)制作脓毒症休克大鼠模型,各实验组相应处理,酶联免疫(ELISA)法检测治疗前后血液中血红蛋白清道夫受体(sCD163)、白介素6(IL-6)、降钙素原(PCT)的含量。结果脓毒症休克模型大鼠中,中西医联合治疗组的大鼠一般情况优于B组,且该组大鼠sCD163、IL-6、PCT的含量明显低于单用西药组以及单用中药组,组间比较差异有统计学意义(P<0.05)。结论中西医联合用药治疗能改善大鼠脓毒症休克症状,其中机制可能是通过抑制sCD163、IL-6、PCT的表达,而且sCD163可能可以作为临床中脓毒症休克用药观察的敏感指标。
Objective To explore the influence of Chinese and Western medicine on the sepsis rats and its mechanism of action, and to provide basis for the early diagnosis of sepsis and assess its prognosis. Methods Sixty SPF healthy SD rats were selected and randomly divided into five groups (12 rats in each group), including normal control group (A group), saline control group (B Group C, group D and group E. Group A was given no injection of normal saline and the rest were treated with continuous infusion of lipopolysaccharide (LPS) via the femoral vein ) Were used to make septic shock rat model. The experimental groups were treated accordingly. The levels of hemoglobin scavenger receptor (sCD163), interleukin 6 (IL-6), procalcitonin PCT) content. Results In septic shock rats, the general condition of rats in combination therapy group was better than that in group B, and the content of sCD163, IL-6 and PCT in this group was significantly lower than those in western medicine alone group and single Chinese medicine group There were significant differences between groups (P <0.05). Conclusion The combination of TCM and WM can improve the sepsis symptoms in rats. The mechanism may be through inhibiting the expression of sCD163, IL-6 and PCT, and sCD163 may be used as a sensitive indicator of sepsis in clinical observation.