Techniques to label the neuroretina and the retinal pigment epithelium (RPE) have been the topic of many studies for years. The reliability and reproducibility of these techniques are essential to investigate retinal alterations of ocular pathologies and possible treatments. In particular, the study of the integrity of the RPE is of great importance in pathologies such as retinitis pigmentosa or age-related macular degeneration (Gu et al., 2012;Fisher and Ferrington, 2018;Zhang et al., 2019). The most common approaches use antibodies to identify different cell populations. Recently, we have described a novel technique to study the integrity of the neuroretina and the RPE, as well as the functionality of the RPE in rats (Valiente-Soriano et al., 2020).