【摘 要】
：
为了解单核细胞增生李斯特菌(Listeria monocytogenes LM.)新疆野毒株XS5 SigmaB操纵子的分子生物学特征.对LM-XS5的SigmaB操纵子部分基因序列进行了克隆与序列分析,预测该操
【机 构】
：
石河子大学新疆地方与民族高发病教育部重点实验室,中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室
论文部分内容阅读
为了解单核细胞增生李斯特菌(Listeria monocytogenes LM.)新疆野毒株XS5 SigmaB操纵子的分子生物学特征.对LM-XS5的SigmaB操纵子部分基因序列进行了克隆与序列分析,预测该操纵子各基因编码蛋白质的二三级结构及功能活性位点,分析其同源性.结果表明:成功扩增出3000bp的SigmaB操纵子片段,序列分析显示该片段中包含RsbV、RsbW、RsbX和SigmaB4个基因,它们编码的蛋白质含有不同的功能活性位点,其中RsbV的5～102位AA为“STAS-抗-抗-SigamB因子”,RsbW的43～157位AA间为HATPase_c功能域,RsbX的32～198位AA间为PP2Cc超家族区域,SigmaB的8～264位AA为RNA聚合酶全酶中的SigmaB因子的区域.LM-XS5与其他3种革兰氏阳性菌的SigmaB基因的同源率在50％左右,与LM参考株的同源率在92.18％～100％之间.
其他文献
Biochemical changes of natural actomyosin from fresh pale, soft, exudative (PSE) and normal pork were studied, and the effects of different storage temperatures
Twelve species,including three Hystrix species,five Leymus species,Hordeum bogdanii,Pseudoroegneria spicata,Psathyrostachys huashanica,and Roegneria ciliaris,we
The pollen morphology of 11 species of the genus Glycyrrhiza L.with one from each of the genera Glycyrrhizopsis Boiss.& Bal.and Meristotropis Fisch.& C.A.Mey.wa
按照肟基在甾核中的不同取代位置进行分类,对国内外近年来有关甾体肟类化合物及其衍生物的合成、生理活性研究中具有代表性的工作进行了综述,并对此方面的发展趋势、应用前景
知识溢出效应对项目外包参与组织获取竞争优势,提高项目成功率具有重要意义.从知识特性、组织特性、项目特性和项目负责人特性四个层面分析了影响参与组织间知识溢出的关键因
Ionic liquids have negligibly low vapor pressure, high stability and polarity. They are regarded as green solvents. Enzymes, especially lipases, as well as whol
This paper describes empirical research on the model, optimization and supervisory control of beer fermentation. Conditions in the laboratory were made as simil
Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rar
Phylogeny and infrageneric relationships were reconstructed for Korean Viola(Violaceae)using eight chloroplast sequences: the atpB-rbcL intergenic spacer,the at
Temperature-sensitive hydrogel-poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthe