乙酰水杨酸(ASA)中含有微量的乙酰水杨酸酐(ASN)、乙酰水杨基水杨酸(ASSA)、水杨基水杨酸(SSA)等过敏性杂质。用紫外分光光度法测定其中的ASN,需用2-苯甲酰胺肉桂酸钠,该试剂国内尚无生产,且需用有毒试剂吡啶。本文用高效液相色谱法,可使ASA中的ASN、ASSA、SSA等达到良好的分离(见图1)。用标准添加法或外标法定量测定其中的ASN,得到满意的结果。此法专一、灵敏、简便、 Acetylsalicylic acid (ASA) contains traces of allergic impurities such as acetylsalicylic anhydride (ASN), acetylsalicylic acid (ASSA) and salicylsalicylic acid (SSA). Determination of ASN by UV spectrophotometry, the need for 2-benzamide sodium cinnamate, the reagent is still no domestic production, and the need to use toxic reagents pyridine. In this paper, high performance liquid chromatography, ASA ASA, ASSA, SSA, etc. to achieve a good separation (see Figure 1). The ASN was determined quantitatively by standard addition method or external standard method, and satisfactory results were obtained. This method is specific, sensitive, simple,