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建立了畜禽粪便中3种β-受体激动剂药物残留的超高压液相色谱-串联四极杆质谱联用仪测定方法。样品酶解后经高氯酸溶液净化,调节p H值至碱性,乙酸乙酯提取,净化浓缩后以流动相定容,用超高压液相色谱-质谱联用仪检测,内标法定量。3种β-受体激动剂在0.5~20.0μg/L范围内线性关系良好,相关系数均大于0.998。畜禽粪便中3种β-受体激动剂的加标回收率为80.3%~110.2%,相对标准偏差为3.0%~7.7%,检出限(S/N=3)为0.04~0.07μg/kg。
A method for determination of three kinds of β-agonist drug residues in livestock and poultry by ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry was established. Samples were hydrolyzed by perchloric acid solution, p H value was adjusted to basicity, extracted with ethyl acetate, purified and concentrated to the mobile phase volume, detected by ultra-high pressure liquid chromatography-mass spectrometry, internal standard method . Three kinds of β-agonists in the range of 0.5 ~ 20.0μg / L good linearity, the correlation coefficient was greater than 0.998. The recoveries of three β-agonists in livestock and poultry manure were 80.3% -110.2% and the relative standard deviations were 3.0% -7.7%. The detection limit (S / N = 3) was 0.04-0.07 μg / kg.