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目的探讨茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制。方法 SD雌性大鼠50只,随机分为正常对照组(10只)和奥氮平诱导肥胖模型组(40只)。肥胖模型组于黑暗时相前1h奥氮平1.2 mg·kg~(-1)·d~(-1)灌胃,正常对照组给予等容量蒸馏水,连续2 wk。于wk 3开始,再将肥胖模型大鼠随机分为4组:模型组、西布曲明(2.5 mg·kg~(-1)·d~(-1))组、茶多酚低(150 mg·kg~(-1)·d~(-1))剂量组和茶多酚高(300 mg·kg~(-1)·d~(-1))剂量组,每组10只。连续给药5 wk。观察大鼠体重、肾和子宫周围脂肪湿重,计算Lee’s指数和脂肪系数,测定血清瘦素、脂联素水平以及超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与正常对照组相比,模型组大鼠体重和脂肪湿重及Lee’s指数和脂肪系数均增加,血清瘦素水平升高,脂联素水平降低(P<0.05)。与模型组比较,茶多酚低、高剂量组大鼠体重和脂肪湿重降低,Lee’s指数、脂肪系数降低,SOD活性增加,MDA含量减少(P<0.05)。与模型组比较,茶多酚高剂量组血清脂联素水平升高(P<0.05),瘦素水平无显著差异(P>0.05)。结论茶多酚对奥氮平诱导的肥胖大鼠有减肥作用,其作用机制可能与增强脂联素的调节和提高抗氧化能力有关。
Objective To investigate the effect of tea polyphenols on weight loss in olanzapine-induced obese rats and its mechanism. Methods Fifty female SD rats were randomly divided into normal control group (n = 10) and olanzapine-induced obesity model group (n = 40). Obese rats were orally administered with olanzapine 1.2 mg · kg -1 · d -1 at 1 h before dark phase, and normal rats were given equal volume of distilled water for 2 weeks. At the beginning of wk 3, the obese rats were randomly divided into 4 groups: model group, sibutramine group (2.5 mg · kg -1 · d -1) mg · kg -1 · d -1 and high dose of tea polyphenols (300 mg · kg -1 · d -1), 10 rats in each group. Administered continuously for 5 weeks. The body weight, wet weight of fat around kidney and uterus were observed. The Lee’s index and fat coefficient were calculated. The levels of serum leptin, adiponectin, superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Results Compared with the normal control group, body weight and wet weight of fat, Lee’s index and fat coefficient of model rats increased, serum leptin level increased and adiponectin level decreased (P <0.05). Compared with the model group, body weight and wet weight of fat in the low and high dose groups of tea polyphenols decreased, the Lee’s index, the fat coefficient decreased, the SOD activity increased and the MDA content decreased (P <0.05). Compared with the model group, the level of serum adiponectin in high-dose tea polyphenols group was increased (P <0.05), while there was no significant difference in leptin level (P> 0.05). CONCLUSION Tea polyphenols has a weight-loss effect on olanzapine-induced obese rats, and its mechanism may be related to enhancing the regulation of adiponectin and enhancing the anti-oxidative capacity.