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以抗大肠癌单抗Ｈｂ３进行免疫亲和层析从大肠癌细胞株ＨＲＴ－１８细胞提取溶液中纯化相应的大肠癌相关抗原ＣＡ－Ｈｂ３，用ＳａｎｄｗｉｃｈＥＬＩＳＡ方法对大肠癌及消化道其他肿瘤患者血清中ＣＡ－Ｈｂ３水平进行定量检测。结果表明，大肠癌阳性率为６８．９％，胃癌阳性率为５６．７％，消化道良性疾病的阳性率为１１．９％。ＣＡ－Ｈｂ３对胃肠道肿瘤诊断的敏感性和特异性较高，是具有应用价值的新型大肠癌血清标志物。
Anti-colorectal monoclonal antibody Hb3 was used for immunoaffinity chromatography to purify the corresponding colorectal cancer-associated antigen CA-Hb3 from the colorectal cancer cell line HRT-18 cell extract, and serum ELISA was used to detect colorectal cancer and other tumors of the digestive tract. Quantitative detection of CA-Hb3 levels. The results showed that the positive rate of colorectal cancer was 68.9%, the positive rate of gastric cancer was 56.7%, and the positive rate of benign diseases of the digestive tract was 11.9%. CA-Hb3 has high sensitivity and specificity for the diagnosis of gastrointestinal tumors and is a new serum marker of colorectal cancer with application value.