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目的:探讨槲皮素对小鼠前额叶皮质(prefrontal cortex,PFC)锥体神经元动作电位的效应。方法:成年C57小鼠或Thy1-GCaMP 3.0小鼠,制作含PFC的冠状脑片。分成正常脑脊液孵育组(对照组)和槲皮素孵育组(50μmol/L),给予高钾(5 mmoL/L KCl)人工脑脊液灌流两组脑片记录GCaMP荧光信号,全细胞膜片钳记录两组PFC锥体神经元放电,碘化丙啶(propidium iodide,PI)染色检测神经元活性。结果:5 mmol/L KCl人工脑脊液可以明显引起对照组GCaMP脑片PFC锥体神经元荧光信号增强,而对槲皮素组无明显作用。全细胞膜片钳记录显示槲皮素组神经元能够产生动作电位。但与对照组相比,产生放电所需刺激强度增加,连续放电能力降低。PI染色显示槲皮素组神经元死亡数目减少。结论:槲皮素孵育可以通过抑制小鼠前额叶皮质锥体神经元产生动作电位的能力,从而提高神经元在体外环境的生存能力。其具体的离子通道机制及细胞信号通路研究需进一步的深入研究。
Objective: To investigate the effect of quercetin on the action potentials of pyramidal neurons in mouse prefrontal cortex (PFC). Methods: Adult C57 mice or Thy1-GCaMP 3.0 mice were used to prepare coronal slices containing PFC. The rats were divided into two groups: normal cerebrospinal fluid (control group) and quercetin group (50μmol / L), and GCaMP fluorescence signal was recorded by perfusion with high potassium (5 mmol / L KCl) PFC pyramidal neurons were discharged and propidium iodide (PI) staining was used to detect neuronal activity. Results: 5 mmol / L KCl artificial cerebrospinal fluid could obviously increase the fluorescence signal of PFC neurons in GCaMP brain slices of control group, but had no effect on quercetin group. Whole-cell patch-clamp recordings showed that quercetin group neurons were able to produce action potentials. However, compared with the control group, the intensity of the stimulus needed to generate the discharge increased and the continuous discharge capacity decreased. PI staining showed a decrease in the number of neuronal deaths in the quercetin group. CONCLUSION: Quercetin can increase neuronal survival in vitro by inhibiting the production of action potential in mouse prefrontal cortical pyramidal neurons. The specific ion channel mechanism and cell signaling pathways need further study.