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目的探讨重组基质溶解素(recombinantmatrilysin,rMMP-7)和脂质体-MMP-7反义寡核苷酸(antisenseoli-gonucleotideofmatrilysinbyliposometransfection,LIPO-MAON)对肺腺癌A549细胞和人脐静脉血管内皮细胞(humanum-bilicalveinendothelialcell,HUVEC)增殖的影响。方法①构建MMP-7的反义寡核苷酸。②MTT法检测rMMP-7和LI-PO-MOAN作用下A549和HUVEC的增殖状况。③RT-PCR法检测rMMP-7和LIPO-MAON作用下腺癌A549细胞和HU-VECMMP-7mRNA的表达。结果0.75、1.0μg/mlrMMP-7对HUVEC增殖有促进作用,各种浓度的LIPO-MAON无作用;对A549细胞的增殖,0.5、0.75、1.0μg/ml的rMMP-7均有促进作用;而高浓度(7.5、10nmol/ml)的LIPO-MAON对A549细胞增殖有明显抑制作用。RT-PCR检测结果表明,HUVEC不表达MMP-7mRNA;rMMP-7能增加A549细胞MMP-7mRNA的表达,LIPO-MAON可降低其MMP-7mRNA的表达(P<0.05)。结论在本试验浓度范围内高浓度的MMP-7能促进正常血管内皮细胞和肺腺癌细胞的增殖,其反义寡核苷酸不能抑制正常血管内皮细胞增殖,但高浓度的反义寡核苷酸能抑制肺腺癌细胞的增殖。
Objective To investigate the effect of rMMP-7 and antisenseoli-gonucleotideofmatrilysinbyliposometransfection (LIPO-MAON) on lung adenocarcinoma A549 cells and human umbilical vein endothelial cells humanum-bilicalveinendothelialcell, HUVEC). Methods ① Construction of antisense oligonucleotide of MMP-7. ② MTT method was used to detect the proliferation of A549 and HUVEC under the action of rMMP-7 and LI-PO-MOAN. ③ RT-PCR was used to detect the expression of HU-VECMMP-7 mRNA in A549 cells and rMMP-7 and LIPO-MAON cells. Results 0.75,1.0μg / ml rMPMP-7 can promote the proliferation of HUVEC, no effect of various concentrations of LIPO-MAON; on the proliferation of A549 cells, 0.5,0.75,1.0μg / ml of rMMP-7 have a promoting effect; High concentration (7.5, 10nmol / ml) of LIPO-MAON A549 cell proliferation was significantly inhibited. RT-PCR results showed that HUVEC did not express MMP-7 mRNA; rMMP-7 increased the expression of MMP-7 mRNA in A549 cells; LIPO-MAON reduced the expression of MMP-7 mRNA (P <0.05). Conclusion High concentrations of MMP-7 promote the proliferation of normal vascular endothelial cells and lung adenocarcinoma cells in the concentration range of the test. Antisense oligonucleotides can not inhibit the proliferation of normal vascular endothelial cells. However, high concentration of antisense oligonucleotides Glyoxal can inhibit the proliferation of lung adenocarcinoma cells.