OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress.METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the procedure.In vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods.RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP treatment.The abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP treatment.More apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell injury.CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury. OBJECTIVE Ganoderma lucidum polysaccharide peptides (GLPP) have an anti-oxidant activity. Oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury (RIRI). The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress .METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group, 100 mg · kg -1 · d -1 of GLPP were injected intraperitoneally For 7dbefore the procedure.In vitro, NRK-52 Ecells subjected to hypoxia-reoxygenation (H / R) and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods .RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular n ecrosis, brush border loss, cast formation, vacuolization and tubular dilatation while these injuries were significantly attenuated by GLPP treatment. The abnormal levels of MPO, MDA and SOD caused by renal ischemia- in the renal ischemia-reperfusion group than the sham group received GLPP reduced apoptotic cells in the ischemia-reperfusion mice by 21.75% (P <0.01). GLPPs (25-1 μg · mL) alleviated H / R induced cell viability loss by 20.12% (P <0.01) and Δφm dissipation by 27.3% (P <0.01) in vitro as well and its pretreatment of dramatically reduced H / R and tunicamycin induced cell injury. CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti -oxidative capacity, which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury.