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选择经冠脉造影证实的冠心病患者16例和健康对照者8例,抽提RNA,筛选冠心病相关差异基因,构建冠心病差异基因表达谱,并使用实时荧光定量逆转录聚合酶链式反应(RT-PCR)对目标基因进行鉴定.另按原标准筛选冠心病患者30例和健康对照者40例,验证目标基因IL-8的临床相关性.在此基础上,以12例健康自愿者为对象,研究IL-8对血小板聚集率、血小板活化和血小板聚集形态学的影响.结果表明,冠心病相关的差异基因共有107个,其中分子功能和生物学途径涉及炎症免疫反应的有14个(13.1%);通路显著性分析发现,冠心病相关有意义通路共15个,其中有4个涉及炎症免疫反应.目标基因鉴定结果与基因芯片检测结果一致.冠心病患者血清IL-8水平(83.21±34.33pg/mL)显著高于健康对照者(63.34±36.36pg/mL)(P<0.05).血小板聚集率、血小板活化和血小板聚集形态学结果显示,IL-8具有诱导血小板活化的作用.本文从核酸水平揭示了冠心病与炎症免疫反应的相关性,目标基因IL-8可能通过影响血小板的活化程度而参与了冠心病的发病过程.
16 patients with coronary heart disease confirmed by coronary angiography and 8 healthy controls were selected and RNA was screened for differentially expressed genes related to coronary heart disease to construct differential gene expression profiles of coronary heart disease. Real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) to identify the target gene.Another 30 cases of coronary heart disease patients and 40 healthy controls were screened according to the original standard to verify the clinical relevance of the target gene IL-8.Furthermore, 12 healthy volunteers As the object, to study the effect of IL-8 on the rate of platelet aggregation, platelet activation and platelet aggregation morphology.The results showed that there were 107 differential genes related to coronary heart disease, including 14 molecular and biological pathways involving inflammatory and immune responses (13.1%). Significance analysis of pathways revealed that there were 15 pathways related to coronary heart disease, of which 4 involved inflammatory and immune responses.The results of target gene identification were consistent with the results of microarray detection.Serum levels of IL-8 in patients with coronary heart disease 83.21 ± 34.33pg / mL) was significantly higher than that of the healthy controls (63.34 ± 36.36pg / mL) (P <0.05) .Immunopreservation of platelet aggregation, platelet activation and platelet aggregation showed that IL- Effect. Disclosed herein coronary heart disease-related immune response to inflammation from the nucleic acid level, the gene of IL-8 may affect the degree of activation by platelets and is involved in the pathogenesis of coronary heart disease.