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AIM:To investigate the effects of dendritic cells(DCs)transfected with full-length wild-type p53 and stimulatedby gastric cancer lysates on immune response.METHODS:The wild-type p53was transduced to DCs withadenovirus,and the DCs were stimulated by gastric cancerlysates.The surface molecules(B7-1,B7-2,MHC-Ⅰ,MHC-Ⅱ)of all DCs were detected by FACS,and the ability of the DCsto induce efficient and specific immunological response inanti-~(51)Cr-labeled target cells was studied.BALB/c miceinjected with DCs and Mk28 were established,and CTLresponse in mice immunized with Lywt-p53DC was evaluated.Tumor-bearing mice were treated with Lywt-p53DC.RESULTS:The surface molecules of Lywt-p53DC had a highexpression of B7-1(86.70±0.07%),B7-2(18.77±0.08%),MHC-Ⅰ(87.20±0.05%)and MHC-Ⅱ(56.70±0.07%);Tlymphocytes had a specific CTL lysis ability induced by Lywt-p53DC;the CTL lysis rate was as high as 81%.The immuneprotection of Lywtp-53DC was obvious,the tumor diameterin Lywtp-53DC group was 3.10±0.31 mm,2.73±0.23mm,3.70±0.07 mm on d 13,16 and 19,respectively,whichwere smaller than control,DC,wtp53DC and LyDC group(P<0.05).Tumor growth rate in Lywtp53DC group wasslower than that in other groups(P<0.05).CONCLUSION:DCs transfected with wild-type p53 andstimulated by gastric cancer lysates have specific CTL killingactivity.
AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response. METHODS: The wild-type p53was transduced to DCs withadenovirus, and the DCs were stimulated by gastric cancerlysates The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCsto induce efficient and specific immunological response in anti- target cells were studied. BALB / c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53 DC was evaluated. Tumor-bearing mice were treated with Lywt-p53 DC. RESULTS: The surface molecules of Lywt-p53 DC had a Tlymphocytes had a specific CTL lysis ability induced by highexpression of B7-1 (86.70 ± 0.07%), B7-2 (18.77 ± 0.08%), MHC-Ⅰ (87.20 ± 0.05%) and MHC-Ⅱ by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immuneprotection of Lywtp-53DC was obvious, the tumor diameterin Lywtp-53DC group was 3.1 0 ± 0.31 mm, 2.73 ± 0.23 mm, 3.70 ± 0.07 mm on d 13, 16 and 19, respectively, whichwere smaller than control, DC, wtp53DC and LyDC group (P <0.05) .Tumor growth rate in Lywtp53DC group wasslower than that in other groups (P <0.05) .CONCLUSION: DCs transfected with wild-type p53 andstimulated by gastric cancer lysates have specific CTL killingactivity.