近年来,随着对微血管和内皮细胞生理病理研究的迅速发展,体外培养内皮细胞和建立毛细血管模型成为亟待解决的问题之一。国内外对于培养单层内皮细胞的经验已较成熟,但要在体外形成立体的毛细血管结构的工作国内尚未见报道。我们成功地建立了大鼠脑毛细血管体外立体培养的方法,现介绍如下。 In recent years, with the rapid development of the research on the physiology and pathology of capillaries and endothelial cells, culturing endothelial cells in vitro and establishing capillary models have become urgent problems to be solved. At home and abroad for the training of monolayer endothelial cells have more mature experience, but in vitro formation of three-dimensional structure of the capillary has not been reported in the country. We have successfully established a method of rat brain capillary in vitro culture, are described below.