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目的:比较高效液相色谱法(HPLC)和荧光偏振免疫法(FPIA)测定艾司唑仑中毒患者的血药浓度的结果,为临床准确测定其血药浓度提供参考。方法:采用HPLC法和FPIA法分别测定了71份艾司唑仑中毒患者的血标本,并对两种方法的测定结果作了统计学分析。结果:两种方法的测定结果无显著性差异(P>0.05),相关性较好,回归方程为Y(FPIA测定值)=1.127X(HPLC测定值)+0.056 7,r=0.989。结论:HPLC法和FPIA法均能用于监测艾司唑仑中毒患者的血药浓度,能较准确地反映中毒原因及中毒的严重程度,为临床制定抢救方案提供客观依据。
OBJECTIVE: To compare the plasma concentrations of eszolam poisoning patients with those of high-performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA), and to provide a reference for the clinical accurate determination of plasma concentrations. Methods: The blood samples of 71 patients with eszolaurin were determined by HPLC and FPIA respectively. The results of two methods were statistically analyzed. Results: There was no significant difference between the two methods (P> 0.05), and the correlation was good. The regression equation was Y (FPIA value) = 1.127X (HPLC value) +0.056 7, r = 0.989. Conclusion: Both HPLC and FPIA can be used to monitor the blood concentration of eszolam poisoning patients, which can accurately reflect the cause of poisoning and the severity of poisoning, and provide an objective basis for the development of clinical rescue plan.