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目的探讨无小牛血清培养外周血淋巴细胞制备微核的可行性。方法采用常规淋巴细胞微核培养法,分别配制两种培养液(有小牛血清培养液和无小牛血清培养液),取静脉血约0.5 ml于无菌条件下接种于培养液中,放置培养箱内培养72 h后收获细胞,低渗液处理、固定液固定、常规制片,Giemsa染色,然后进行阅片和计数。结果有小牛血清培养液实验组与无小牛血清培养液实验组(χ2=0.769,P>0.05)的淋巴细胞微核率相比较,差异没有统计学意义;有小牛血清培养液实验组与有小牛血清培养液对照组(χ2=37.863,P<0.05)、无小牛血清培养液实验组与无小牛血清培养液对照组(χ2=39.178,P<0.05)的淋巴细胞微核率相比较,差异均有统计学意义。结论采用无小牛血清培养外周血淋巴细胞制备微核是可行的。
Objective To investigate the feasibility of culturing micronuclei in peripheral blood lymphocytes without calf serum. Methods The method of routine lymphocyte micronucleus culture was used to prepare two kinds of culture medium (with bovine serum culture medium and no calf serum culture medium). About 0.5 ml venous blood was inoculated into the culture medium under sterile conditions and placed The cells were harvested after incubating for 72 h in the incubator, treated with hypotonic solution, fixed with fixative, routinely prepared and stained with Giemsa, then read and counted. Results There was no significant difference between the experimental group and the experimental group without calf serum (χ2 = 0.769, P> 0.05). There was no significant difference between the experimental group and the experimental group (Χ2 = 37.863, P <0.05), and there was no difference between the control group (χ2 = 37.863, P <0.05) and the control group without the serum of calf serum Compared with the rates, the differences were statistically significant. Conclusion It is feasible to culture micronuclei using peripheral blood lymphocytes without calf serum.