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肥大细胞在过敏性疾病发生中起关键作用,而RBL-2H3细胞系作为大鼠嗜碱性粒细胞系(rat basophilic leukemia,RBL)的一个亚系,可以表达肥大细胞的许多特性和功能,因此常作为过敏性疾病研究的替代模型。RBL-2H3细胞系常用作过敏反应中肥大细胞脱颗粒和激活机制方面的研究,此外,该细胞系在抗原特异性IgE的检测、过敏原的分离鉴定和临床过敏性疾病诊断等方面也有较高的应用价值。本文重点综述了RBL-2H3细胞系在过敏性疾病研究的多个领域中的应用情况。
Mast cells play a key role in the development of allergic diseases, whereas the RBL-2H3 cell line, as a sub-line of the rat basophilic leukemia (RBL), expresses many of the features and functions of mast cells and therefore Often used as an alternative to allergic disease research. The RBL-2H3 cell line is often used as a study on the mechanism of degranulation and activation of mast cells in anaphylaxis. In addition, the cell line is also high in the detection of antigen-specific IgE, the isolation and identification of allergens and the diagnosis of clinical allergic diseases The application value. This article highlights the use of the RBL-2H3 cell line in several areas of allergic disease research.