目的:观察四君子汤对脾虚大鼠唾液淀粉酶分泌障碍治疗效果,并揭示其作用机制。方法:大鼠皮下注射利血平0.5 mg/kg,正常对照组大鼠注射等量生理盐水,共8 d;造模结束后模型动物分为模型组和四君子汤组,四君子汤组灌胃给予四君子汤,模型组和正常对照组灌胃给予等量双蒸水,给药4 w后进行取材。麻醉动物,取左侧腮腺,缝合伤口,大鼠稍清醒时,用10%冰乙酸溶液20μL刺激大鼠舌尖,1次/min,共刺激30次;处死,取下右侧腮腺。检测腮腺组织唾液淀粉酶活性(sAA)c、AMP及VIP含量、PKA活性和VAMP8、SNAP-23蛋白表达。结果:四君子汤组大鼠腮腺内sAA与正常对照组大鼠无显著差异,模型组大鼠酸刺激后sAA活性显著高于正常对照组大鼠,酸刺激前VIP含量、PKA活性各组大鼠无显著差异;酸刺激后,正常对照组大鼠VIP及cAMP含量、PKA活性显著升高,模型组大鼠VIP轻微升高,cAMP含量、PKA活性显著低于正常对照组,四君子汤组大鼠VIP及cAMP含量、PKA活性有一定程度恢复。模型组VAMP-8蛋白表达与正常对照组比较无显著差异,SNAP-23蛋白表达有所下降;与模型组比较,四君子汤组大鼠SNAP-23、VAMP-8蛋白表达增强。结论:四君子汤对利血平脾虚大鼠唾液淀粉酶分泌障碍有一定疗效,可能通过恢复脾虚大鼠腮腺细胞VIP-cAMP信号通路改变起到治疗作用,包括提高VIP含量和提高SNAP-23和VAMP-8蛋白表达。 Objective: To observe the therapeutic effect of Sijunzi Decoction on salivary amylase secretion disorder in spleen deficiency rats and to reveal its mechanism. Methods: The rats were injected subcutaneously with reserpine 0.5 mg / kg, and the rats in the normal control group were injected with the same amount of normal saline for 8 days. After the model was established, the model animals were divided into model group and Sijunzi Decoction group. Sijunzi Decoction, model group and normal control group were given the same amount of double-distilled water, administered 4 w after drawing. Animals were anesthetized, the left parotid gland was taken and the wounds were sutured. When rats were slightly awake, the tongue of rat was stimulated with 20 μL of 10% glacial acetic acid solution for 1 time / min for 30 times. The animals were sacrificed and the right parotid gland was removed. The salivary amylase activity (sAA) c, AMP and VIP, PKA activity and the expression of VAMP8 and SNAP-23 in the parotid gland were detected. Results: sAA in the parotid gland of Sijunzi Decoction group showed no significant difference compared with that of the normal control group. The sAA activity of the model group was significantly higher than that of the normal control group There was no significant difference between the two groups. After acid stimulation, VIP and cAMP content and PKA activity in the normal control group were significantly increased. VIP in the model group increased slightly, cAMP content and PKA activity were significantly lower than those in the normal control group VIP and cAMP content, PKA activity to some extent restored. Compared with normal control group, the expression of VAMP-8 protein in model group had no significant difference, and SNAP-23 protein expression decreased. Compared with model group, SNAP-23 and VAMP-8 protein expression increased in Sijunzi Decoction group. Conclusion: Sijunzi Decoction has certain therapeutic effect on salivary amylase secretion in reserpine-deficient rat model of Spleen Deficiency, which may play a therapeutic role by restoring the VIP-cAMP signal pathway in parotid gland cells of rats with spleen deficiency syndrome, including increasing VIP content and increasing SNAP-23 and VAMP -8 protein expression.