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目的观察纳米银粒子、异噻唑啉酮的抗大肠杆菌效果。方法按照倍比稀释法配备纳米银粒子、异噻唑啉酮溶液，按照不同浓度加1ml到琼脂中均匀，制备平板，0浓度做空白对照组，接种大肠杆菌，观察细菌生长情况。结果肉眼观察实验组纳米银粒子浓度9.6μg/mL、异噻唑啉酮浓度2.5%时，未见大肠细菌生长，而空白对照组的菌落生长明显。结论纳米银粒子，异噻唑啉酮在混合琼脂平板中，在适当浓度时，能抑制大肠杆菌生长，更详细的应用有待深入实验研究。“,”Objective To observe the ef ect of silver nanoparticles against Escherichia coli, isothiazolone. Methods According to the double dilution method with silver nanoparticles, isothiazolone solution, according to the dif erent 1ml concentration to agar uniform, preparation of tablet, 0 concentration of the blank control group, inoculated with Escherichia coli, observe the growth of bacteria. Results Macroscopic observation of silver nanoparticles concentration experimental group 9.6 g/mL, isothiazolinones at the concentration of 2.5%, no coliform bacteria growth, while in the control group the colony growth significantly. Conclusion Nano silver particles, isothiazolone in mixed agar, at appropriate concentrations, can inhibit the growth of Escherichia coli, further experimental study on the application of more detaile.