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以高抗疫病的甜椒育种材料‘N1345’与高感疫病辣椒材料‘N1308’为双亲,构建了6个联合世代,运用植物数量性状主基因+多基因联合分离分析方法对N1345疫病抗性进行遗传分析,并对其F2群体进行EST-SSR与SSR连锁标记分析。结果显示,N1345对疫病抗性由2对加性—显性—上位性主基因控制(B-1-1),两主基因加性效应、显性效应均相等,主基因遗传率在B1、B2和F2世代分别为63.43%、82.32%和83.46%。筛选到E73、E318等2个EST-SSR标记与疫病抗性病级均呈显著负相关,相关系数分别为﹣0.6296、﹣0.5492,差异水平均为极显著(P<0.01),表明这2个标记与对疫病抗性基因紧密连锁。这2个标记在22份甜辣椒材料中分离一致,有6份材料抗、感表型与标记分离不一致。
Six joint generations were constructed with parents of pepper breeding material ’N1345’ and highly susceptible pepper material ’N1308’ which were highly resistant to disease. The resistance of N1345 was analyzed by the combined analysis of major gene + polygene of plant quantitative traits Genetic analysis and EST-SSR and SSR linkage analysis of their F2 population. The results showed that the resistance of N1345 was controlled by two pairs of additive-dominance-epistatic major genes (B-1-1). The additive and dominance effects of the two major genes were equal. The heritability of the major genes was in B1, B2 and F2 generations were 63.43%, 82.32% and 83.46% respectively. The two EST-SSR markers, E73 and E318, were significantly and negatively correlated with the disease resistance level, the correlation coefficients were -0.6296 and -0.5492, respectively, and the difference was significant (P <0.01), indicating that these two markers Closely linked to the disease resistance genes. The two markers were separated in 22 sweet pepper materials, with 6 materials resistant. The phenotypic and marker separation was inconsistent.