Objective: To verify the antidiabetic activity of leaf extracts from Physalis peruviana L. popularly used in the East part of the Democratic Republic of the Congo and to point out the possible toxicity.Method:pigs at the dose range of 100 mg/kg to 3.2 g/kg of body weight. The hypoglycemic activity was evaluated by glucose tolerance test, loading animals with glucose 4 g/kg and measuring blood glucose concentrations at various times. The effect was compared to the control and glibenclamide as antidiabetic reference drug. Acute toxicity was evaluated by recording mortality rate, changes on blood biomarkers and damage caused to vital organs.Results:At a dose of 100 mg/kg, the aqueous extract induced a significant reduction of peak Aqueous decoctions prepared from dried leaves powder were administrated to guinea concentration at 30 min after glucose loading as compared with control or reference (P<0.05). At doses greater than 400 mg, some alterations on blood, kidney and liver markers were observed. Upper 800 mg/kg, mortality was observed with LD50 estimated at about 1280 mg/kg. At the autopsy, vital organs were in haemorrhage and swelling state.Conclusion:The crude aqueous extracts from the leaves of Physalis peruviana L. present hypoglycemic activity in animal model, but at high doses the plant may cause severe intoxication.