近年在研究药理动力学方面,要求制定一种能在生物体液中同时分别定量测定完整的青霉素及其代谢产物的分析方法。过去测定α-氨基青霉素(氨苄霉素及羟氨苄青霉素)的萤光法能满足此要求,但其缺点是仅能测定对-α-氨基青霉素,而不能测定很多其它青霉素。本文报道一种新的灵敏萤光法,可用以同时测定完整的青霉素及其生物转化产物(青霉噻唑酸)。本法系 In recent years in the study of pharmacokinetics, requires the development of a biological fluid can be simultaneously quantitative analysis of intact penicillin and its metabolites analysis. In the past, the fluorimetric method for the determination of a-aminopenicillin (ampicillin and amoxicillin) fulfilled this requirement, but the disadvantage was that only alpha-aminopenicillin could be assayed and that many other penicillins could not be assayed. This article reports a new sensitive fluorescence method that can be used to simultaneously determine intact penicillin and its bioconversion product (penicillin). This law