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目的 :检测 Ki- 6 7抗原在膀胱移行细胞癌 (TCC)中的表达 ,研究肿瘤增生活性与肿瘤生物学行为的关系。方法 :应用免疫组化 SP法检测 45例膀胱 TCC和 12例正常膀胱组织 Ki- 6 7抗原的表达。Ki- 6 7标记指数 (Ki- 6 7L I)指染色阳性细胞占全部细胞的百分数。其分级标准为 :无阳性细胞为— ,阳性细胞 <2 5 %为 +,≥ 2 5 %、<5 0 %为 ++,≥ 5 0 %、<75 %为 +++,≥ 75 %为 ++++。结果 :Ki- 6 7抗原阳性表达率膀胱 TCC组为 48.47% ,对照组为 8.33% (P=0 .0 11) ;临床分期 Ta～ T1 为 35 .71% ,T2 ～ T4为 76 .47% ,病理分级 G1 为 2 5 % ,G2 为 5 3.33% ,G3 为 90 %。随着肿瘤分期分级升高 ,Ki- 6 7L I逐渐上升 (P=0 .0 0 4,P=0 .0 0 1)。Ki- 6 7L I肿瘤复发、多发者明显高于初发、单发者 (P=0 .0 14,P=0 .0 34 )。结论 :细胞无限增生是肿瘤形成的重要原因 ,Ki- 6 7L I能准确地评估膀胱 TCC的生物学行为 ,可作为膀胱 TCC有重要意义的肿瘤标志物
Objective: To detect the expression of Ki-67 antigen in transitional cell carcinoma of bladder (TCC) and to study the relationship between tumor proliferative activity and tumor biological behavior. Methods: Immunohistochemical SP method was used to detect the expression of Ki- 6 7 in 45 cases of bladder TCC and 12 cases of normal bladder tissues. The Ki- 6 7 labeling index (Ki- 6 7L I) refers to the percentage of stained positive cells to total cells. The grading standards were: no positive cells -, positive cells <25% +, ≥ 25%, <50% ++, ≥ 50%, <75% +++, 75% ++++. Results: The positive rate of Ki- 6 7 antigen was 48.47% in the bladder TCC group and 8.33% in the control group (P = 0.011). The clinical stage Ta ~ T1 was 35.71%, the T2 ~ T4 was 76.47% The pathological grading G1 was 25%, G2 was 5 3.33%, and G3 was 90%. As the tumor staging increased, Ki- 6 7L I gradually increased (P = 0.004, P = .0 0 1). The recurrence rate of Ki- 6 7L I in patients with multiple tumors was significantly higher than that in those with primary or single tumors (P = 0.014, P = 0.034). Conclusion: Infinite hyperplasia of cells is an important cause of tumor formation. Ki- 6 7L I can accurately evaluate the biological behavior of bladder TCC and may be used as a tumor marker of bladder TCC