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Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor.Transcriptional responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana;however,corresponding data aren’t available for non-heading Chinese cabbage.To address this problem,we describe a suppression subtractive library-based approach to isolate the plant’s ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases,70 up-regulated genes were classified into 9 parts:some aspect of primary ’metabolism’ or ’energy’ production;’protein synthesis’ or ’protein fate’;cellular communication/signal transduction mechanism;cell fates including Beclin,SPT1,and SPT2;HLA-B and AGOI which participate in transcription;cellular transport and hypothetical proteins or proteins for which a function has yet to be determined.Seven selected genes such as Beclin,thioredoxin,HLA-B,MAP3K,SPT1,SPT2,and AGO1 were up-regulated induced by PB90,suggesting that the genes may play an important role in PB90-triggered HR.
Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. Description response to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana; however, corresponding data are not available for non-heading Chinese cabbage. To address this problem, we describe a suppression subtractive library-based approach to isolate the plant’s ESTs up-regulated in the early induction / execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genomes databases, 70 up-regulated genes were classified into 9 parts: some aspect of primary ’metabolism’ or ’energy ’production;’ protein synthesis’ or ’protein fate’; cellular communication / signal transduction mecha HLA-B and AGOI which participate in transcription; cellular transport and hypothetical proteins or proteins for which a function has yet to be determined. Selected genes such as Beclin, thioredoxin, HLA- B, MAP3K, SPT1, SPT2, and AGO1 were up-regulated by PB90, suggesting that the genes may play an important role in PB90-triggered HR.