目的利用近红外光谱技术,快速测定丹参药材中丹参酮ⅡA的含量。方法运用偏最小二乘法(PLS)建立丹参药材中丹参酮ⅡA含量与其近红外光谱之间的校正模型,并对未知的丹参样品进行丹参酮ⅡA含量预测。结果校正集内部交叉验证决定系数R2为0.996 94,交互验证均方根偏差RMSECV为0.011 8,11份样品的外部预测均方根偏差RMSEP为0.023 7。预测值的平均相对误差为1.712 9%。结论近红外光谱技术测定丹参药材中丹参酮ⅡA的含量是可行的,该方法快速准确、简单无损,可应用于大批量样品的快速分析。 Objective To rapidly determine the content of tanshinone ⅡA in Radix Salviae Miltiorrhizae by near-infrared spectroscopy. Methods Partial least square method (PLS) was used to establish a calibration model between the content of tanshinone Ⅱ A and its near infrared spectra in Radix salviae miltiorrhizae, and the prediction of the content of tanshinone Ⅱ A in Salvia miltiorrhiza samples was carried out. Results The internal cross-validation coefficient R2 of the calibration set was 0.99694, RMSECV of cross-validation was 0.011 8, RMSEP of external forecast root mean square deviation of samples was 0.023 7. The average relative error of the predicted value is 1.712 9%. Conclusion It is feasible to determine the content of tanshinone ⅡA in Radix Salviae Miltiorrhizae by near infrared spectroscopy. The method is rapid and accurate, simple and nondestructive, and can be applied to the rapid analysis of large quantities of samples.