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本研究以抗逆性强的大豆旱碱一号为材料,首次从中克隆了编码9-顺式-环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase,NCED)的GmNCED1基因全长cDNA片段,该基因编码区含有1 836个核苷酸,编码611个氨基酸。通过同源性比对分析发现GmNCED1与花生、菜豆等双子叶豆科植物NCED的同源性高达84%以上。同时,利用荧光定量PCR分析发现高盐、低温、干旱、外源ABA以及NAA处理均可以诱导该基因在大豆叶片及根中表达。本研究初步揭示GmNCED1基因在植物逆境胁迫中的作用,为基因工程育种提供优质的候选基因。
In this study, the full-length GmNCED1 gene encoding 9-cis-epoxycarotenoid dioxygenase (NCED) was cloned for the first time using ascorbic acid cDNA fragment, the gene coding region contains 1 836 nucleotides, encoding 611 amino acids. Homology comparison showed that the homology between GmNCED1 and NCED of dicotyledonous legumes such as peanut and kidney bean was more than 84%. At the same time, high-temperature, low-temperature, drought, exogenous ABA and NAA treatments could all induce the expression of this gene in soybean leaves and roots. In this study, we first revealed the role of GmNCED1 in plant stress response and provided excellent candidate genes for genetic engineering breeding.