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为评估自日本引进的抗性黑松对松材线虫(Pine Wood Nematode,PWN)的抗病性,选用3个不同来源的PWN强毒和中强毒虫株采用皮接法接种,对1.5年生的黑松13个优良家系进行抗病性测定。结果表明,引进的黑松大部分家系对3个PWN虫株都表现出一定的抗病性,但针对不同虫株,抗病各家系间差异明显,其中有7个家系(3、4、5、6、9、11和12号)表现良好。同时,为探讨引进黑松家系感染PWN后的组织病理反应,对12号抗性家系进行组织病理学研究。观察表明,松材线虫在抗性黑松和普通黑松中的纵向扩散速度差别不明显,但其在抗性黑松内的横向扩散速度比在普通黑松内要慢,且抗性黑松体内木射线细胞和围绕树脂道泌脂细胞的薄壁细胞,出现病变要比普通黑松晚;在接种后同一时间取茎段的同一部位发现,抗性松苗组织解剖结构及细胞的病变和破坏程度要比普通松苗轻。
To evaluate the resistance of the resistant black pine to pine wood nematode (PWN) introduced from Japan, 3 virulent and moderately virulent strains of PWN from 3 different sources were inoculated by skinning, 13 excellent families of black pine were tested for disease resistance. The results showed that most of the pedigrees introduced had some disease resistance to three PWN strains, but there was significant difference among the resistant strains for different strains, of which 7 families (3, 4, 5, 6, 9, 11 and 12) performed well. At the same time, in order to investigate the histopathological response after the introduction of PWN in the black pine family, the 12th resistant pedigree was studied by histopathology. The observation showed that there was no obvious difference in the vertical diffusion rate of pine wood nematode among resistant black pine and common black pine, but the horizontal diffusion rate in resistant black pine was slower than that in common black pine and that in resistant black pine Wood-ray cells and parenchyma cells around the resin-derived sebaceous cells showed lesion later than common black pine. At the same time after inoculation, the same site of the stems was observed. The anatomic structure of the resistant pine seedlings and the extent of the lesions and destruction of the cells Light pine than ordinary.