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目的:通过建立实验性自身免疫性脑脊髓炎(EAE)动物模型,给予神经生长子(NGF)治疗,检测NGF对EAE神经干细胞增殖的影响。方法:以豚鼠脑脊髓匀浆加完全弗氏佐剂作为抗原,给予Wistar大鼠四足垫皮内注射,建立EAE模型,对正常组,免疫后7天组,发病后3天、7天、14天及21天组和治疗组给予NGF治疗EAE,同时分别进行5-溴脱氧尿嘧啶核苷(BrdU)免疫组织化学染色,以观察NGF促神经干细胞增殖的效应。结果:与EAE组比较,治疗组BrdU阳性细胞表达明显增强,治疗组与EAE组之间在发病后7天、14天和21天差异均有统计学意义(P<0.05)。且NGF对小儿病毒性脑炎有显著临床疗效。结论:NGF治疗能够促进EAE大鼠脑内神经干细胞增殖,对小儿脑部疾病的研究有指导意义。
OBJECTIVE: To establish an animal model of experimental autoimmune encephalomyelitis (EAE) and to observe the effects of NGF on the proliferation of EAE neural stem cells treated with nerve growth factor (NGF). Methods: The guinea pig spinal cord homogenate plus complete Freund’s adjuvant was used as antigen. Wistar rats were injected intradermally with four-foot pad. EAE model was established. Normal group, 7 days after immunization, 3 days, 7 days after onset, 14 days and 21 days group and treatment group were given NGF treatment of EAE, at the same time were 5-bromodeoxyuridine (BrdU) immunohistochemical staining to observe the effect of NGF on neural stem cell proliferation. Results: Compared with EAE group, the expression of BrdU positive cells in the treatment group was significantly increased (P <0.05). The differences between the treatment group and the EAE group were statistically significant at 7 days, 14 days and 21 days after onset. And NGF has a significant clinical effect on viral encephalitis in children. Conclusion: NGF treatment can promote the proliferation of neural stem cells in the brain of EAE rats, which is instructive for the study of pediatric brain diseases.