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[目的]探讨六味地黄汤对非酒精性脂肪性肝病大鼠固醇调节元件结合蛋白(SREBP-1c)和肝X受体(LXR-α)的影响。[方法]选择雄性SD大鼠,随机分3组:正常组,六味地黄汤组(治疗组)和模型组。正常组喂养普通饲料,模型组和治疗组喂养高脂饲料建立非酒精性脂肪性肝病模型,治疗组并用六味地黄汤干预治疗;荧光定量RT-PCR检测各组大鼠SREBP-1c和LXR-α的基因表达。[结果]模型组大鼠SREBP-1cmRNA和LXR-αmRNA的相对表达均高于正常组(分别为t=2.361,P<0.05和t=3.118,P<0.01);与模型组相比,治疗组能显著降低SREBP-1cmRNA和LXR-αmRNA的表达(分别为t=3.816,P<0.01和t=3.36,P<0.01)。[结论]六味地黄汤能降低SREBP-1c和LXR-α的基因表达,改善脂质代谢。
[Objective] To investigate the effect of Liuwei Dihuang decoction on steroid regulatory element binding protein (SREBP-1c) and liver X receptor (LXR-α) in non-alcoholic fatty liver disease. [Methods] Male SD rats were randomly divided into 3 groups: normal group, Liuwei Dihuang Decoction group (treatment group) and model group. The rats in the normal group were fed with normal feed, the model group and the treatment group were fed with high-fat diet to establish non-alcoholic fatty liver disease model. The treatment group was treated with Liuweidihuang Decoction. Fluorescent quantitative RT-PCR was used to detect the SREBP-1c and LXR-α The gene expression. [Results] The relative expression of SREBP-1 mRNA and LXR-αmRNA in the model group was significantly higher than that in the normal group (t = 2.361, P <0.05 and t = 3.118, P <0.01) Significantly reduced the expression of SREBP-1 mRNA and LXR-α mRNA (t = 3.816, P <0.01 and t = 3.36, P <0.01, respectively). [Conclusion] Liuwei Dihuang Decoction can reduce the gene expression of SREBP-1c and LXR-α and improve lipid metabolism.