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建立了测定大豆和葛根７种异黄酮成分的高效液相色谱法。采用日本岛津Ｓｈｉｍ－Ｐａｒｋ ＣＬＣ ＯＤＳ柱（１５０ ｍｍ ｘ ６．０ ｍｍ ＩＤ；５μｍ），Ｖ 甲醇：Ｖ醋酸： Ｖ水＝ ３０：３．５：６６． ５体系为流动相，０～８． ５ｍｉｎ对流动相流速为 １． ０ ｍＬ／ｍｉｎ，８．５～ ４７ｍｉｎ时流速为１．５ｍＬ／ｍｉｎ，柱温５０℃ ，检测波长 ２５４ｎｍ，外标法定量。
A high performance liquid chromatography (HPLC) method for the determination of 7 isoflavones in soybean and kudzu root was established. A Shimadzu Shim-Park CLC ODS column (150 mm x 6.0 mm ID; 5 μm) from Shimadzu Japan was used. V methanol: V acetic acid: V water = 30: 3.5: 66. 5 system as mobile phase, 0 ~ 8. 5min The mobile phase flow rate of 1. 0 mL / min, flow rate of 1.5 mL / min at 8.5 ~ 47 min, column temperature of 50 ℃, detection wavelength of 254 nm, and external standard method.