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研究将大肠杆菌胞嘧啶脱氨酶（CD）基因应用于基因治疗。构建、包装含CD基因的假型逆转录病毒。构建含CD基因的逆转录病毒重组表达载体pLCDSN基础上，以重组表达载体pLCDSN转染包装细胞PA317，pLCDSN整合入PA317染色体中。结果：CD基因获得表达且包装出具有感染能力的假型逆转录病毒。以该假型逆转录病毒感染NIH／3T3细胞，逆转录病毒重组表达载体pLCDSN整合入NIH／3T3细胞染色体中，CD基因获得表达。5－FC对有CD基因表达的包装细胞PA317（pLCDSN）和NIH／3T3（pLCDSN）产生杀伤毒性。结论：我们已成功地构建、包装了含重组逆转录病毒载体pLCDSN且具有感染能力的假型逆转录病毒。该工作为以逆转录病毒介导的CD基因应用于肿瘤基因治疗奠定了基础。
The study used E. coli cytosine deaminase (CD) gene for gene therapy. Construct and package pseudotyped retroviruses containing the CD gene. On the basis of constructing the retroviral recombinant expression vector pLCDSN containing CD gene, the packaging cell PA317 was transfected with the recombinant expression vector pLCDSN, and pLCDSN was integrated into the PA317 chromosome. Results: The CD gene was expressed and packaged with pseudotyped retroviruses capable of infection. NIH/3T3 cells were infected with the pseudotyped retrovirus and retroviral recombinant expression vector pLCDSN was integrated into the chromosome of NIH/3T3 cells, and the CD gene was expressed. 5-FC produced cytotoxicity against packaging cells PA317 (pLCDSN) and NIH/3T3 (pLCDSN) with CD gene expression. Conclusion: We have successfully constructed and packaged a pseudotyped retrovirus containing the recombinant retroviral vector pLCDSN and capable of infection. This work laid the foundation for retrovirus-mediated CD gene application in tumor gene therapy.