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目的:研究淋巴化疗对胃癌淋巴转移灶的直接治疗作用。方法:实验组(淋巴化疗组):术前经内镜胃粘膜下注射,加手术时胃浆膜下及腹膜内注射化疗药与活性碳的混悬液;对照组(血液化疗组):术前经股动脉插管灌注化疗药的水溶液,用流式细胞术检测化疗前后的胃癌组织及化疗后的转移淋巴结。结果:淋巴化疗后,检测术后阳性淋巴结,其SPF(S期细胞比例)、APO(细胞凋亡)发生率及bcl-2表达与动脉灌注化疗组之间的差异有高度显著性(P<0.01)。结论:经血液途径给药的化疗对淋巴系统转移灶作用甚微,而淋巴化疗能促进淋巴系统肿瘤细胞凋亡的发生,对淋巴转移灶具有直接的治疗作用。
Objective: To study the direct therapeutic effect of lymphatic chemotherapy on lymph node metastasis of gastric cancer. METHODS: The experimental group (lymphoid chemotherapy group): preoperative submucosal submucosal injection plus intraoperative gastric subserosal and intraperitoneal injection of chemotherapeutic drugs and activated carbon suspension; control group (blood chemotherapy group): surgery Anterior aqueous solutions of femoral artery catheters were used to perfuse chemotherapeutic agents. Flow cytometry was used to detect gastric cancer tissues before and after chemotherapy and metastatic lymph nodes after chemotherapy. RESULTS: After lymphatic chemotherapy, the positive rate of postoperative lymph nodes was significantly different between the SPF (S phase cell ratio), the incidence of APO (apoptosis), and the expression of bcl-2 in the arterial infusion chemotherapy group (P< 0.01). Conclusion: Chemotherapy with blood route has little effect on lymphatic system metastasis, and lymphatic chemotherapy can promote lymphocyte apoptosis and has a direct therapeutic effect on lymph node metastases.